专家人才库数据----中国科学院昆明动物研究所

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郑萍研究员

哺乳动物胚胎发育学科组

职　　务：

学　　历：

博士

电　　话：

+86 871 65197853

传　　真：

电子邮件：

zhengp@mail.kiz.ac.cn

通讯地址：

昆明市盘龙区茨坝青松路21号 650201

其他主页：

CASKIZ-IR(机构知识库）个人主页

简　　历

2009.06 至今，中国科学院昆明动物研究所研究员，组建哺乳动物胚胎发育课题组，担任课题组长。

2005.06 — 2009.03，美国国立卫生研究院（NIH）博士后。

2003.03 — 2005.06，美国Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine博士后。

2001.08 — 2003.03，中国科学院昆明动物研究所助理研究员。

1995.09 — 2001.08，中国科学院昆明动物研究所，获博士学位。

1990.09 — 1994.07，重庆师范学院生物系获学士学位。

研究方向

1. 干细胞维持基因组稳定性的特征和分子调控研究

干细胞是机体发育和组织稳态维持的基础，其基因组的稳定维持对正常发育及组织器官的稳态维持至关重要。由于功能特殊，干细胞在基因组稳定性调控上有其独特的特征和机制。我们以胚胎干细胞和神经干细胞为对象，研究干细胞调控基因组稳定性的特殊分子机制。这些研究将有助于推动干细胞的安全应用，并有助于理解相关发育异常和疾病的致病机理。

2. 生殖干细胞的基础和应用研究

雌性生殖细胞的再生研究：哺乳动物雌性配子的发生是否存在再生机制一直是生殖生物学领域长期争议的话题，目前尚没有确凿的证据支持生理状况下存在卵巢生殖干细胞及其再生功能。我们以小鼠为模型，通过遗传手段着重研究生理状态下是否存在卵细胞的再生现象,并研究再生的细胞基础。

雄性生殖干细胞研究：树鼩（类灵长类动物）和猕猴（非人灵长类）与人的亲缘关系接近。我们重点研究树鼩和猕猴的精原干细胞的分子特征和体外长期扩增培养体系，并利用精原干细胞系进行基因修饰，创建疾病动物模型，模拟和研究人类疾病。

3. 灵长类早期胚胎发育

由于伦理原因，人类自身的发育过程一直都非常不清楚，特别是早期胚胎的发育调控。利用云南的灵长类资源，我们开展了猕猴着床前后早期胚胎发育的研究，重点探讨发育多能性调控、早期细胞命运分化及胚层发生。这些研究有望为干细胞及转化研究提供核心理论基础，也有助于理解和防范人类胚胎发育异常和失败。

承担科研项目

1.国家自然科学基金重点项目，多能干细胞高效调控DNA复制压力反应的关键lncRNA鉴定与功能分析, 31930027，2020.01-2024.12，项目主持人

2.国家自然科学基金-云南联合基金重点项目，猴精原干细胞系的建立及基于精原干细胞研发猴高效基因敲入新技术，U2102202，2022/01-2025/12，项目主持人

3.国家“干细胞研究与器官修复”重点专项，灵长类多能干细胞基因组稳态调控网络及增强策略，2021YFA1102000，2021/12-2026/06, 项目主持人（首席科学家）

专家类别

研究员

社会任职

获奖及荣誉

代表论著

代表性工作（*通讯作者）

1. Jiang F^#, Wang L^#, Dong Y, Nie W, Zhou H, Gao J, Zheng P*. DPPA5A suppresses the mutagenic TLS and MMEJ pathways by modulating the cryptic splicing of Rev1 and Polq in mouse embryonic stem cells. Proc Natl Acad Sci USA, 2023, 120(30):e2305187120.

2. Gong D, Wang L, Zhou H, Gao J, Zhang W, Zheng P*. Long noncoding RNA Lnc530 localizes on R-loops and regulates R-loop formation and genomic stability in mouse embryonic stem cells. Stem Cell Reports, 2023, 18(4):952-968.

3. Ma H^#, Ning Y^#, Wang L^#, Zhang W, Zheng P*. Lnc956 regulates mouse embryonic stem cell differentiation in response to DNA damage in a p53-independent pathway. Science Advances, 2023, 9(3):eade9742.

4. Zhang W^#, Tang M^#, Wang L, Zhou H, Gao J, Chen Z, Zhao B*, Zheng P*. Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis. Science Advances, 2023, 9(4):eadf6277.

5. Zheng P. Current understanding of genomic stability maintenance in pluripotent stem cells. Acta biochimica et biophysica Sinica, 2022, 54(6), 858-863. (invited review)

6. Qin DD^#, Zhou JK^#, He XC^#, Shen XY^#, Li C, Chen HZ, Yan LZ, Hu ZF, Li X, Lv LB, Yao YG*, Wang Z*, Huang XX*, Hu XT*, Zheng P*. Depletion of giant ANK2 in monkeys causes drastic brain volume loss. Cell Discovery, 2021, 7(1):113.

7. Wang L^#, Li J^#, Zhou H, Zhang W, Gao J, Zheng P*. A novel lncRNA Discn fine-tunes replication protein A (RPA) availability to promote genomic stability. Nature Communications, 2021,12(1):5572.

8. Li JZ, Shang YF, Wang L, Zhao B, Sun CL, Li JL, Liu SL, Li C, Tang M, Meng FL*, Zheng P*. Genome integrity and neurogenesis of postnatal hippocampal neural stem/progenitor cells require a unique regulator Filia. Science Advances, 2020, 6(44):eaba0682.

9. Ma H^#, Zhai J^#, Wan H^#, Jiang X^#, Wang X, Wang L, Xiang Y, He X, Zhao ZA, Zhao B, Zheng P*, Li L*, Wang H*. In vitro culture of cynomolgus monkey embryos beyond early gastrulation. Science, 2019, 366(6467):eaax7890.

10. Zhang WD^#, Chen ZL^#, Zhang DF, Zhao B, Liu L, Xie ZY, Yao YG, Zheng P*. KHDC3L mutation causes recurrent pregnancy loss by inducing genomic instability of human early embryonic cells. PLoS Biology, 2019, 17(10):e3000468.

11. Luo X^#, He Y^#, Zhang C^#, He X^#, Yan L^#, Li M, Hu T, Hu Y, Jiang J, Meng X, Ji W, Zhao X, Zheng P*, Xu S*, Su B*. Trio deep-sequencing does not reveal unexpected off-target and on-target mutations in Cas9-edited rhesus monkeys. Nature Communications, 2019, 10(1):5525.

12. Liu D^#, Wang X^#, He D^#, Sun C^#, He X, Yan L, Li Y, Han JJ*, Zheng P*. Single-cell RNA-sequencing reveals the existence of naive and primed pluripotency in pre-implantation rhesus monkey embryos. Genome Research, 2018, 28 (10):1481–1493. (封面论文)

13. Zhao B^#, Zhang WD^#, Cun YX, Li JZ, Liu Y, Gao J, Zhu HW, Zhou H, Zhang RG, Zheng P*. Mouse embryonic stem cells have increased capacity for replication fork restart driven by the specific Filia-Floped protein complex. Cell Research, 2018, 28(1):69-89.

14. Fan Y^#, Luo R^#, Su L-Y, Xiang Q, Yu D, Xu L, Chen J-Q, Bi R, Wu D-D, Zheng P*, Yao Y-G*. Does the genetic feature of the Chinese tree shrew (Tupaia belangeri chinensis) support its potential as a viable model for Alzheimer’s disease research?Journal of Alzheimers Disease, 2018, 61(3):1015-1028.

15. Wang XY^#, Liu DH^#, He DJ, Suo SB, Xia X, He XC, Han JJ*, Zheng P*. Transcriptome analyses of rhesus monkey pre-implantation embryos reveal a reduced capacity for DNA double strand break (DSB) repair in primate oocytes and early embryos. Genome Research, 2017, 27(4):567-579.

16. Li CH, Yan LZ, Ban WZ, Tu Q, Wu Y, Wang L, Bi R, Ji S, Ma YH, Nie WH, Lv LB, Yao YG*, Zhao XD*, Zheng P*. Long-term propagation of tree shrew spermatogonial stem cells in culture and successful generation of transgenic offspring. Cell Research, 2017, 27(2):241-252.

17. Guo K^#, Li CH^#, Wang XY, He DJ, Zheng P*. Germ stem cells are active in postnatal mouse ovary under physiological conditions. Molecular Human Reproduction, 2016, 22(5):316-328 (该工作受到杂志同期特邀评论, Mol Hum Reprod, 2016, 22:313-315).

18. Lu YQ, He XC, Zheng P*. Decrease in expression of maternal effect gene Mater is associated with maternal ageing in mice. Molecular Human Reproduction, 2016, 22(4):252-260.

19. Zhao B, Zhang WD, Duan YL, Lu YQ, Cun YX, Li CH, Guo K, Nie WH, Li L, Zhang R, Zheng P*. Filia is an ESC-specific regulator of DNA damage response and safeguards genomic stability. Cell Stem Cell, 2015, 16(6):684-698.

20. Zhao YQ^#, Ji S^#, Wang JK^#, Huang JF*, Zheng P*. mRNA-Seq and microRNA-Seq whole-transcriptome analysis of rhesus monkey ESC neural differentiation revealed the potential regulators of rosette neural stem cells. DNA Research, 2014, 21(5):541-54.

21. Zheng P*, Baibakov B, Wang XH, Dean J*. PtdIns(3,4,5)P3 is constitutively synthesized and required for spindle translocation during meiosis in mouse oocytes. Journal of Cell Science, 2013, 126(Pt 3):715-21.

22. Li L, Zheng P, Dean J*. Maternal control of early mouse development. Development, 2010, 137:859-870. (review)

23. Zheng P, Dean J*. Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage stage mouse embryogenesis. Proc Natl Acad Sci USA, 2009, 106(18):7473-8.

24. Zheng P, Vassena R, Latham KE*. Effects of in vitro oocyte maturation and embryo culture on the expression of glucose transporters, glucose metabolism and insulin signaling genes in rhesus monkey oocytes and preimplantation embryos. Molecular Human Reproduction, 2007, 13:361-371.

25. Zheng P*, Dean J. Oocyte-specific genes affect folliculogenesis, fertilization, and early development. Seminars in Reproductive Medicine, 2007, 25(4):243-251. (Invited review)

26. Zheng P*. Effects of in vitro maturation of monkey oocytes on their developmental capacity. Animal Reproduction Science, 2007, 98:56-71. (Invited review)

27. Zheng P, Vassena R, Latham KE*. Expression and downregulation of WNT signaling pathway genes in rhesus monkey oocytes and embryos. Molecular Reproduction and Development, 2006, 73:667-677.

28. Zheng P, Patel B, McMenamin M, Moran E, Paprocki AM, Kihara M, Schramm RD, Latham KE*. Effects of follicle size and oocyte maturation conditions on maternal message RNA regulation and gene expression in rhesus monkey oocytes and embryos. Biology of Reproduction, 2005, 72:890-897.

29. Zheng P, Schramm RD, Latham KE*. Developmental regulation and in vitro culture effects on expression of DNA repair and cell cycle checkpoint control genes in rhesus monkey oocytes and embryos. Biology of Reproduction, 2005, 72:1359-1369.

30. Zheng P, Patel B, McMenamin M, Reddy, S, Paprocki AM, Schramm RD, Latham KE*. The primate embryo gene expression resource: A novel resource to facilitate rapid analysis of gene expression patterns in non-human primate oocytes and preimplantation stage embryos. Biology of Reproduction, 2004, 70:1411-1418.

31. Zheng P, Patel B, McMenamin M, Paprocki AM, Schramm RD, Nagl N, Wilsker D, Wang G, Moran E, Latham KE*. Expression of genes encoding chromatin regulatory factors in developing rhesus monkey oocytes and preimplantation stage embryos: Possible roles in genome activation. Biology of Reproduction, 2004, 70:1419-1427.

32. Zheng P, Si W, Bavister BD, Yang JF, Ding CH, Ji WZ*. 17-b Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys. Human Reproduction, 2003, 18(10):2137-2144.

33. Zheng P, Bavistser BD, Ji WZ*. Amino acid requirements for maturation of rhesus monkey oocytes in culture. Reproduction, 2002, 124, 515-525.

34. Zheng P, Wang H, Bavister BD, Ji WZ*. Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessmental by IVF and embryo development. Human Reproduction, 2001, 16(2):300-305.

35. Zheng P, Bavister BD, Ji WZ*. Energy substrate requirement for in vitro maturation of oocytes from unstimulated adult rhesus monkeys. Molecular Reproduction and Development, 2001, 58: 348-355.

36. Zheng P, Si W, Wang H, Zou RJ, Bavister BD, Ji WZ*. Effect of age and breeding season on the developmental capacity of oocytes from unstimulated and FSH-stimulated rhesus monkeys. Biology of Reproduction, 2001, 64:1417-1421.

研究团队

工作人员：王林（副研究员）、马怀孝（副研究员）、张伟道（副研究员）、林慧娟（特别研究助理）

研究生：

博士研究生：李聪、金洁、唐敏、谢恒、董玉萍、李超

硕士研究生：李羿霏、谢澜萍、施文、吴含、钟婉欢