| 作者: |
Jianbo Li, Ligang Wang, Dawei Yu, Junfeng Hao, Longchao Zhang, Adeniyi C Adeola, Bingyu Mao, Yun Gao, Shifang Wu, Chunling Zhu, Yongqing Zhang, Jilong Ren, Changgai Mu, David M Irwin, Lixian Wang, Tang Hai, Haibing Xie, Yaping Zhang |
| 摘要: |
Thoracolumbar vertebra (TLV) and rib primordium (RP) development is a common evolutionary feature across vertebrates, although whole-organism analysis of TLV and RP gene expression dynamics has been lacking. Here we investigated the single-cell transcriptomic landscape of thoracic vertebra (TV), lumbar vertebra (LV), and RP cells from a pig embryo at 27 days post-fertilization (dpf) and identified six cell types with distinct gene-expression signatures. In-depth dissection of the gene-expression dynamics and RNA velocity revealed a coupled process of osteogenesis and angiogenesis during TLV and rib development. Further analysis of cell-type-specific and strand-specific expression uncovered the extremely high levels of Hoxa10 3'-untranslated region (UTR) sequence specific to osteoblast of LV cells, which may function as anti-Hoxa10-antisense by counteracting the Hoxa10-antisense effect to determine TLV transition. Thus, this work provides a valuable resource for understanding embryonic osteogenesis and angiogenesis underlying vertebrate TLV and RP development at the cell-type-specific resolution, which serves as a comprehensive view on the transcriptional profile of animal embryo development |
